3PIM
Crystal structure of Mxr1 from Saccharomyces cerevisiae in unusual oxidized form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-15 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 111.894, 111.894, 108.124 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.23754 |
| Rwork | 0.236 |
| R-free | 0.26448 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pil |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 59060 | |
| Completeness [%] | 95.6 | 97.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 289 | 0.5M ammonium sulfate, 0.1M sodium citrate, 1.0M lithium sulfate, pH 5.6, VAPOR DIFFUSION, temperature 289K |
