3PHW

OTU Domain of Crimean Congo Hemorrhagic Fever Virus in complex with Ubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	77
Detector technology	CCD
Collection date	2009-10-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	79.440, 106.170, 111.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.623 - 2.000
R-factor	0.2164
Rwork	0.213
R-free	0.27830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.903
Data scaling software	SCALA
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.4_122))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.000
Number of reflections	59820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.27 M sodium phosphate dihydrogen, 1.53 M di-potassium phosphate hydrogen, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K