3PHV

X-RAY ANALYSIS OF HIV-1 PROTEINASE AT 2.7 ANGSTROMS RESOLUTION CONFIRMS STRUCTURAL HOMOLOGY AMONG RETROVIRAL ENZYMES

Experimental procedure

Spacegroup name	P 41 21 2
Unit cell lengths	50.240, 50.240, 107.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.700
R-factor	0.191 *
Rwork	0.191
RMSD bond length	0.017
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.700 *
Rmerge	0.110 *
Total number of observations	10000 *
Number of reflections	2200 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6 (mg/ml)
2	1	drop	imidazole/HCl	100 (mM)	or NaMOPS/MOPS
3	1	drop		250 (mM)
4	1	drop	DTT	10 (mM)
5	1	drop		3 (mM)