3PH6
Bovine beta lactoglobulin crytsallized through ligandation of yttrium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-06-24 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.060, 63.710, 134.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.640 - 2.530 |
| R-factor | 0.21722 |
| Rwork | 0.216 |
| R-free | 0.26520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.023 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.580 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Rmerge | 0.097 | 0.650 |
| Number of reflections | 21458 | 1522 |
| <I/σ(I)> | 17 | 3 |
| Completeness [%] | 95.0 | 92.4 |
| Redundancy | 7.7 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch crystallization | 7 | 277 | 0.3 mM Yttrium, pH 7, Batch crystallization, temperature 277K |
