3PH1
The Crystal Structure of a Pathogenic Protein from the Xanthomonas campestris Reveals a New Tetrameric PilZ Domain Self-Assembled via a Unusual Helical Bundle
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97973 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.840, 89.360, 87.340 |
| Unit cell angles | 90.00, 105.40, 90.00 |
Refinement procedure
| Resolution | 25.020 - 2.100 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.25900 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.190 |
| High resolution limit [Å] | 2.100 | 2.110 |
| Number of reflections | 101046 | |
| Completeness [%] | 90.0 | 98.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 298 | 20% PEG 3350, pH 6, VAPOR DIFFUSION, temperature 298K |
