3PG8

Truncated form of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase from Thermotoga maritima

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	110
Detector technology	CCD
Collection date	2010-03-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	53.510, 55.456, 166.935
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.570 - 2.000
R-factor	0.158
Rwork	0.155
R-free	0.20940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rzm
RMSD bond length	0.006
RMSD bond angle	0.995
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	PHENIX (1.6.1_357)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.047	45.047	2.110
High resolution limit [Å]	2.000	6.320	2.000
Rmerge		0.038	0.293
Rmeas		0.044	0.337
Rpim		0.021	0.162
Total number of observations		3914	17558
Number of reflections	32385
<I/σ(I)>	12.3	28.8	4.1
Completeness [%]	94.5	88.2	96.3
Redundancy	3.7	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293.15	8%(w/v) PEG 4000, 0.1M sodium acetate, 0.02%(w/v) sodium azide, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K