3PG1

MAP kinase LmaMPK10 from Leishmania major (1.95 angs resolution)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	108
Detector technology	IMAGE PLATE
Collection date	2010-08-04
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	81.260, 81.260, 129.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.730 - 1.950
R-factor	0.1936
Rwork	0.192
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.070
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.15)
Phasing software	AMoRE
Refinement software	BUSTER-TNT

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.730	28.730	2.060
High resolution limit [Å]	1.950	6.170	1.950
Rmerge		0.018	0.537
Total number of observations		2863	15881
Number of reflections	31659
<I/σ(I)>	15.8	30.4	1.4
Completeness [%]	98.3	85.9	97.4
Redundancy	3.5	2.9	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	12% PEG 4000, 0.1M Hepes, 4% isopropanol, 5% glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K