3PFQ
Crystal Structure and Allosteric Activation of Protein Kinase C beta II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-04 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03318 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 114.270, 114.270, 170.840 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 57.100 - 4.000 |
| R-factor | 0.2445 |
| Rwork | 0.193 |
| R-free | 0.24450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 2I0E and 1A25 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.878 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.100 | 4.220 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Number of reflections | 10670 | |
| Completeness [%] | 93.9 | 88 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 294 | 0.1M Tris, pH 8.5, 3% PEG8K, VAPOR DIFFUSION, temperature 294K |
