3PEY

S. cerevisiae Dbp5 bound to RNA and ADP BeF3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.116
Spacegroup name	P 21 21 21
Unit cell lengths	42.136, 92.206, 104.524
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.467 - 1.401
R-factor	0.1612
Rwork	0.160
R-free	0.17950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.516
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.450
High resolution limit [Å]	1.400	3.020	1.400
Rmerge	0.040	0.018	0.435
Number of reflections	76028
<I/σ(I)>	26.2
Completeness [%]	94.0	99.5	67
Redundancy	3.6	3.9	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	20% PEG 3350, 200 mM MgNO3, 10 mM HEPES pH 7.5, 100 mM NaCl, 1mM DTT, 0.5 mM IP6, 5 mM MgCl2, 1 mM ADP, 3 mM BeCl2, 15 mM NaF, 5% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 291K