3PCR
Structure of EspG-Arf6 complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-16 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97956, 0.97989, 0.97188 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 104.566, 104.566, 98.238 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.001 - 2.500 |
| R-factor | 0.2278 |
| Rwork | 0.223 |
| R-free | 0.32640 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.265 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 6.780 | 2.500 |
| Rmerge | 0.050 | 0.029 | 0.830 |
| Number of reflections | 34977 | ||
| <I/σ(I)> | 15.9 | ||
| Completeness [%] | 99.8 | 97 | 100 |
| Redundancy | 9.6 | 8.1 | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 2% PEG 4000, 0.1 M sodium acetate, 5% 2,3-methylpentanediol, pH 5.0, vapor diffusion, hanging drop, temperature 293K |
