3PA6
Structure of the N-terminal BRCT domain of human microcephalin (MCPH1)
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-10 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 35.069, 39.274, 106.639 |
Unit cell angles | 90.00, 91.52, 90.00 |
Refinement procedure
Resolution | 35.530 - 1.500 |
R-factor | 0.146 |
Rwork | 0.144 |
R-free | 0.19000 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.339 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL2Map |
Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.530 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.059 | 0.431 |
Number of reflections | 41554 | |
<I/σ(I)> | 46.1 | 1.9 |
Completeness [%] | 88.9 | 43.1 |
Redundancy | 6.4 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 295 | 0.2M sodium succinate, 15% PEG 3350, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 295K |