3P8V
Crystal structure of the archaeal asparagine synthetase A complexed with L-Aspartic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.87300 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.330, 61.500, 155.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.293 - 1.800 |
| R-factor | 0.1775 |
| Rwork | 0.176 |
| R-free | 0.21810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.882 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 51846 | |
| Completeness [%] | 98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 100mM Tris-HCl pH7, 0.2M NaCl and 32% PEG3350 and soak in 1 to 5mM aspartic acid, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
