3P8D
Crystal structure of the second Tudor domain of human PHF20 (homodimer form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-17 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.992349 |
| Spacegroup name | P 43 |
| Unit cell lengths | 48.548, 48.548, 96.149 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.540 - 2.000 |
| R-factor | 0.2301 |
| Rwork | 0.228 |
| R-free | 0.26220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.654 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.540 | 2.150 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.058 | 0.315 |
| Number of reflections | 14859 | |
| <I/σ(I)> | 31.8 | 3.6 |
| Completeness [%] | 99.3 | 95.6 |
| Redundancy | 8.1 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 12% PEG8000, 0.1 M Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
