3P83
Structure of the PCNA:RNase HII complex from Archaeoglobus fulgidus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 149.670, 152.030, 90.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.540 - 3.050 |
| R-factor | 0.1882 |
| Rwork | 0.187 |
| R-free | 0.21590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1RXM and PDB ENTRY 1I39 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.080 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.9.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 44.540 |
| High resolution limit [Å] | 3.010 |
| Rmerge | 0.116 |
| Number of reflections | 39748 |
| <I/σ(I)> | 15.8 |
| Completeness [%] | 100.0 |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.2 | 293.15 | 18% PEG 3350, 196mM K2HPO4, pH 9.2, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K |
