3P7H

Structure of the human Langerin carbohydrate recognition domain in complex with maltose

Replaces:  3BC6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-11-15
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9796
Spacegroup name	P 42
Unit cell lengths	79.970, 79.970, 90.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.300
R-factor	0.18318
Rwork	0.181
R-free	0.22863
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3p7g
RMSD bond length	0.012
RMSD bond angle	1.360
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.400
High resolution limit [Å]	2.300	2.300
Rmerge		0.188
Number of reflections	25136
<I/σ(I)>	25.6	7.3
Completeness [%]	98.5	98.3
Redundancy	4.1	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.9	293	Protein: 10mg/ml Langerin in 10 mM Tris/HCl pH 7.5, 5 mM Maltose; Reservoir: 0.1 M Na-cacodylate, 13 %(w/v) PEG4000, 0.1 M MgCl2, 5mM CaCl2, VAPOR DIFFUSION, SITTING DROP, temperature 293K