3P6A
Crystal Structure of the DH/PH domains of p115-RhoGEF (R399E mutant)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-10-08 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | P 32 |
| Unit cell lengths | 110.823, 110.823, 98.565 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.300 - 2.500 |
| R-factor | 0.228 |
| Rwork | 0.226 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3odo |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.012 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.091 | 0.580 |
| Number of reflections | 46979 | |
| <I/σ(I)> | 22.7 | 2.1 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 3.6 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 293 | 2.7-3.0M sodium formate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
