3P63

Structure of M. laminosus Ferredoxin with a shorter L1,2 loop

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-06
Detector	ADSC QUANTUM 4r
Wavelength(s)	0.976
Spacegroup name	P 41
Unit cell lengths	58.997, 58.997, 55.898
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.720 - 2.300
R-factor	0.22266
Rwork	0.219
R-free	0.28751
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rfk
RMSD bond length	0.015
RMSD bond angle	1.703
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall
Low resolution limit [Å]	58.720
High resolution limit [Å]	2.100
Number of reflections	10318
Completeness [%]	97.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	293	19% PEG 4000, 0.1M Tris buffer pH 7.2, 0.1M-0.18M Mg(NO3)2, VAPOR DIFFUSION, HANGING DROP, temperature 293K