3P5T

CFIm25-CFIm68 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-03
Detector	MAR CCD 165 mm
Spacegroup name	C 1 2 1
Unit cell lengths	160.440, 105.690, 147.080
Unit cell angles	90.00, 112.72, 90.00

Refinement procedure

Resolution	15.000 - 2.700
R-factor	0.21632
Rwork	0.214
R-free	0.26543
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cl3 2fy1
RMSD bond length	0.011
RMSD bond angle	1.326
Data reduction software	d*TREK
Data scaling software	SCALA
Phasing software	PHASES
Refinement software	REFMAC (5.4.0067)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.768
High resolution limit [Å]	2.700	2.700
Number of reflections	58226
Completeness [%]	98.9	98.92

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	283	16% PEG 3350, 5% dioxane, 0.1M sodium citrate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 283K