3P5N
Structure and mechanism of the S component of a bacterial ECF transporter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0750 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.434, 94.244, 115.402 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.210 - 3.600 |
| R-factor | 0.266 |
| Rwork | 0.265 |
| R-free | 0.28920 |
| Structure solution method | MAD |
| RMSD bond length | 0.039 |
| RMSD bond angle | 1.870 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.6_289) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.730 |
| High resolution limit [Å] | 3.600 | 7.750 | 3.600 |
| Rmerge | 0.065 | 0.029 | 0.831 |
| Number of reflections | 6783 | ||
| <I/σ(I)> | 9.8 | ||
| Completeness [%] | 99.7 | 97.5 | 100 |
| Redundancy | 6.9 | 6.2 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 298 | pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 298 | pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
