3P59
First Crystal Structure of a RNA Nanosquare
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 173 |
| Detector technology | PIXEL |
| Collection date | 2010-04-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9202752 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 62.378, 62.378, 126.268 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.059 - 2.179 |
| R-factor | 0.157 |
| Rwork | 0.156 |
| R-free | 0.19200 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.114 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.060 | 2.270 |
| High resolution limit [Å] | 2.157 | 2.157 |
| Rmerge | 0.065 | 0.330 |
| Number of reflections | 25640 | |
| <I/σ(I)> | 49.5 | 2 |
| Completeness [%] | 86.7 | 30 |
| Redundancy | 30.2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 150 mM magnesium chloride, 50 mM sodium cacodylate, 10mM hexamminecobalt (III) chloride, 50 mM KCl, 17% PEG 400, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
