3P4R

Crystal structure of Menaquinol:fumarate oxidoreductase in complex with glutarate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Wavelength(s)	1.03
Spacegroup name	P 21 21 21
Unit cell lengths	96.678, 138.016, 269.558
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 3.050
R-factor	0.249
Rwork	0.248
R-free	0.28400
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.800
High resolution limit [Å]	2.700	5.810	2.700
Rmerge	0.111	0.084	0.394
Number of reflections	88348
<I/σ(I)>	20.4
Completeness [%]	88.3	94.5	67.1
Redundancy	2.6	3.2	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.8	298	14.5% PEG 8000 mme, 125mM MgAc, 95mM Citrate pH 5.8, 0.1% w/v DTT, 0.1mM EDTA, 10mM glutarate, VAPOR DIFFUSION, temperature 298K