3P4M
Crystal Structure of H2-Kb in complex with the NP205-LCMV epitope YTVKYPNL, an 8-mer peptide from the LCMV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-08-30 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.730, 90.920, 89.050 |
| Unit cell angles | 90.00, 111.09, 90.00 |
Refinement procedure
| Resolution | 19.987 - 2.500 |
| R-factor | 0.1899 |
| Rwork | 0.181 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vac |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.119 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.600 | |
| High resolution limit [Å] | 2.500 | 10.000 | 2.500 |
| Rmerge | 0.082 | 0.021 | 0.231 |
| Rmeas | 0.026 | 0.288 | |
| Number of reflections | 32903 | 427 | 3721 |
| <I/σ(I)> | 10.76 | 26.6 | 4.4 |
| Completeness [%] | 95.3 | 75.2 | 97.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.2M ammonium acetate, 16-24% PEG 3350, 0.1M Na-cacodylate, pH 6.5, vapor diffusion, hanging drop, temperature 277K |
