3P4D
Alternatingly modified 2'Fluoro RNA octamer f/rC4G4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-17 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 41.630, 41.630, 126.489 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 21.080 - 1.850 |
| R-factor | 0.21426 |
| Rwork | 0.210 |
| R-free | 0.27115 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rxb |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.124 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.077 | 0.430 |
| Number of reflections | 3824 | |
| <I/σ(I)> | 28.5 | 6.9 |
| Completeness [%] | 99.5 | 99.7 |
| Redundancy | 13.9 | 14.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | Sodium cacodylate (40 mM, pH 6.0), sodium chloride (80 mM), spermine tetrahydrochloride (12 mM), and 2-methyl-2,4-pentanediol (MPD; 10% (v/v)), equilibrated against a reservoir of MPD (1 mL, 35%), VAPOR DIFFUSION, HANGING DROP, temperature 291K |
