3P34
Polo-like kinase I Polo-box domain in complex with MQSpTPL phosphopeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Collection date | 2009-10-25 |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.184, 77.057, 88.975 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.250 - 1.400 |
| R-factor | 0.21254 |
| Rwork | 0.212 |
| R-free | 0.22958 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1UMW (chain A) |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.048 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 58.380 |
| High resolution limit [Å] | 1.400 |
| Number of reflections | 49803 |
| Completeness [%] | 97.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100mM HEPES, 1.0-2.0M (NH4)2SO4, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
