3P2W
Unliganded form of Polo-like kinase I Polo-box domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Collection date | 2009-08-06 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.462, 98.882, 36.138 |
| Unit cell angles | 90.00, 98.48, 90.00 |
Refinement procedure
| Resolution | 49.450 - 1.660 |
| R-factor | 0.20673 |
| Rwork | 0.205 |
| R-free | 0.23156 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1UMW (chain A) |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.973 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.450 |
| High resolution limit [Å] | 1.660 |
| Number of reflections | 26961 |
| Completeness [%] | 96.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100mM HEPES, 1.0-2.0M (NH4)2SO4, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
