3P2I

Structure of an antibiotic related Methyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	110
Detector technology	CCD
Collection date	2010-03-25
Detector	Bruker Platinum 135
Wavelength(s)	1.541
Spacegroup name	P 43
Unit cell lengths	64.857, 64.857, 108.609
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.400
Rwork	0.205
R-free	0.26560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3pb3
RMSD bond length	0.005
RMSD bond angle	1.156
Data scaling software	SCALEPACK
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.340
High resolution limit [Å]	2.300	6.240	2.300
Rmerge	0.102	0.072	0.256
Number of reflections	19348
<I/σ(I)>	10.8
Completeness [%]	96.5	97.7	76.3
Redundancy	8	11	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.5		pH 5.5