3P26

Crystal structure of S. cerevisiae Hbs1 protein (apo-form), a translational GTPase involved in RNA quality control pathways and interacting with Dom34/Pelota

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.5418
Spacegroup name	P 42 21 2
Unit cell lengths	110.631, 110.631, 188.115
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.087 - 2.500
R-factor	0.2193
Rwork	0.216
R-free	0.27100
Structure solution method	Se-SAD
RMSD bond length	0.006
RMSD bond angle	1.020
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.087	2.540
High resolution limit [Å]	2.500	2.500
Number of reflections	39338
Completeness [%]	95.7	95.69

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	293	100mM Tris-HCl, 12% PEG 6000, 150mM NaCl, pH 8.5, VAPOR DIFFUSION, temperature 293K