3P1M
Crystal structure of human ferredoxin-1 (FDX1) in complex with iron-sulfur cluster
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 32 |
| Unit cell lengths | 76.576, 76.576, 234.410 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.910 - 2.540 |
| R-factor | 0.20541 |
| Rwork | 0.204 |
| R-free | 0.24132 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cje |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.486 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.500 | 2.670 |
| High resolution limit [Å] | 2.540 | 2.540 |
| Rmerge | 0.111 | 0.721 |
| Number of reflections | 50786 | |
| <I/σ(I)> | 11.6 | 2 |
| Completeness [%] | 99.8 | 99 |
| Redundancy | 5.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293.15 | 1.75M Potassium Citrate (K3Cit), pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K |
