3P1J
Crystal structure of human GTPase IMAP family member 2 in the nucleotide-free state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-21 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97895 |
| Spacegroup name | P 65 |
| Unit cell lengths | 132.472, 132.472, 84.161 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.580 |
| R-factor | 0.2062 |
| Rwork | 0.205 |
| R-free | 0.22960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3LXW |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.060 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (BUSTER 2.8.0) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.670 |
| High resolution limit [Å] | 2.580 | 5.550 | 2.580 |
| Rmerge | 0.077 | 0.025 | 0.993 |
| Number of reflections | 26603 | ||
| <I/σ(I)> | 12.9 | ||
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 6.1 | 6.2 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 291 | 5-fold molar excess of GDP was added to protein stock solution. 1.4M sodium citrate, 0.1M HEPES, pH 7.6, vapor diffusion, hanging drop, temperature 291K |
