3P1A
Structure of human Membrane-associated Tyrosine- and Threonine-specific cdc2-inhibitory kinase MYT1 (PKMYT1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-07-09 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.9245 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.400, 56.300, 114.660 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.080 - 1.700 |
R-factor | 0.1522 |
Rwork | 0.150 |
R-free | 0.19495 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 2C30 and 3CR0 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.560 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.320 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.087 | 0.445 |
Number of reflections | 32553 | |
<I/σ(I)> | 13.2 | 3.2 |
Completeness [%] | 98.6 | 95.3 |
Redundancy | 6.2 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293.15 | 20% PEG 3350, 0.2M KSCN, 0.1M Bis-Tris Propane, pH 6.5, 10% Ethylene glycol , VAPOR DIFFUSION, SITTING DROP, temperature 293.15K |