3P12
Crystal Structure of D-ribose Pyranase Sa240
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-03 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9794, 0.9795, 1.0000 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 122.126, 122.126, 175.861 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.290 - 2.350 |
| R-factor | 0.21896 |
| Rwork | 0.217 |
| R-free | 0.25199 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.171 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.110 | 0.485 |
| Number of reflections | 32949 | |
| <I/σ(I)> | 30.6 | 5.6 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 14 | 14.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 281 | 0.1M sodium acetate pH 4.6, 2.0M sodium formate, VAPOR DIFFUSION, HANGING DROP, temperature 281K |
