3P0V
anti-EGFR/HER3 Fab DL11 alone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2008-07-30 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9774 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.375, 77.168, 82.664 |
| Unit cell angles | 90.00, 99.53, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.850 |
| R-factor | 0.22969 |
| Rwork | 0.227 |
| R-free | 0.28018 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.267 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.850 |
| Number of reflections | 20173 |
| <I/σ(I)> | 9.2 |
| Completeness [%] | 100.0 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | MgCl/PEG3350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
