3OZZ
Structure of a cytochrome b5 core-swap mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 80 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-01-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 110.669, 30.654, 23.553 |
| Unit cell angles | 90.00, 95.65, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.700 |
| R-factor | 0.176 |
| Rwork | 0.176 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1awp |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.773 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.050 | 0.152 |
| Number of reflections | 8707 | |
| <I/σ(I)> | 24.5 | 8.2 |
| Completeness [%] | 98.4 | 95.3 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 277 | 30% PEG8000, 0.2M MgAc, 0.1M Pipes, pH 7.0, VAPOR DIFFUSION, temperature 277K |
