3OZE
Crystal Structure of human 5'-deoxy-5'-methyladenosine phosphorylase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-03 |
Wavelength(s) | 1.0809 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 79.517, 81.810, 130.282 |
Unit cell angles | 90.00, 91.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.21848 |
Rwork | 0.216 |
R-free | 0.26243 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.489 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 | 2.070 |
High resolution limit [Å] | 2.000 | 4.300 | 2.000 |
Rmerge | 0.073 | 0.041 | 0.672 |
Number of reflections | 112029 | ||
<I/σ(I)> | 10 | ||
Completeness [%] | 99.6 | 99.4 | 97.6 |
Redundancy | 4 | 4 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 200mM NaCl, 0.1M Hepes, 20% PEG3000, pH 7.5, vapor diffusion, sitting drop, temperature 291K |