3OZ2

Crystal structure of a geranylgeranyl bacteriochlorophyll reductase-like (Ta0516) from Thermoplasma acidophilum at 1.60 A resolution

Replaces:  3CGV

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-01
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97920,0.97862
Spacegroup name	P 21 21 21
Unit cell lengths	49.590, 70.670, 117.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.257 - 1.600
R-factor	0.1539
Rwork	0.153
R-free	0.17060
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.608
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.257	45.257	1.640
High resolution limit [Å]	1.600	7.160	1.600
Rmerge	0.052	0.038	0.466
Number of reflections	55071	648	4009
<I/σ(I)>	13.16	29.4	2.4
Completeness [%]	99.4	89.4	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	15.00% Glycerol, 8.50% iso-Propanol, 17.00% PEG-4000, 0.1M HEPES pH 7.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K