3OXO
Succinyl-CoA:3-ketoacid CoA transferase from pig heart covalently bound to CoA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2007-11-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.11588 |
| Spacegroup name | P 1 |
| Unit cell lengths | 69.121, 107.133, 134.565 |
| Unit cell angles | 89.60, 80.21, 75.13 |
Refinement procedure
| Resolution | 21.200 - 2.300 |
| R-factor | 0.24665 |
| Rwork | 0.245 |
| R-free | 0.27339 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nrb |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.933 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.200 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.086 | 0.237 |
| Number of reflections | 149078 | |
| <I/σ(I)> | 5.9 | 2.3 |
| Completeness [%] | 91.4 | 82.5 |
| Redundancy | 1.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | 20% PEG3350, 100 mM TrisHCl, potassium citrate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
