3OWQ
X-Ray Structure of Lin1025 protein from Listeria innocua, Northeast Structural Genomics Consortium Target LkR164
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-26 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97890 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.510, 157.041, 57.277 |
| Unit cell angles | 90.00, 98.38, 90.00 |
Refinement procedure
| Resolution | 28.332 - 2.606 |
| R-factor | 0.2271 |
| Rwork | 0.225 |
| R-free | 0.25980 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.341 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.600 |
| Rmerge | 0.083 |
| Number of reflections | 58846 |
| <I/σ(I)> | 19.6 |
| Completeness [%] | 99.1 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5), Reservoir solution: LiCl 0.1M, PEG1K 22%, MES 0.1M, VAPOR DIFFUSION, HANGING DROP |
