3OW5
Crystal structure of the Y200A mutant of gamma carbonic anhydrase from Methanosarcina thermophila
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 3 |
Unit cell lengths | 83.498, 83.498, 83.498 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.104 - 1.801 |
R-factor | 0.1296 |
Rwork | 0.129 |
R-free | 0.15170 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.019 |
RMSD bond angle | 1.426 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.6.3_467) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 25.000 | 25.000 | 1.860 |
High resolution limit [Å] | 1.800 | 3.870 | 1.800 |
Rmerge | 0.057 | 0.027 | 0.349 |
Number of reflections | 18286 | ||
<I/σ(I)> | 17.8 | ||
Completeness [%] | 100.0 | 99.9 | 100 |
Redundancy | 6.5 | 6.4 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 5% PEG 8000, with 250 mM ammonium sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |