3OV2
Curcumin synthase 1 from Curcuma longa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2009-12-08 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.180, 115.810, 221.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.820 - 2.320 |
| R-factor | 0.1861 |
| Rwork | 0.183 |
| R-free | 0.23480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.287 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 2.380 | ||
| High resolution limit [Å] | 2.320 | 10.380 | 2.320 |
| Rmerge | 0.098 | 0.039 | 0.421 |
| Rmeas | 0.048 | 0.517 | |
| Number of reflections | 165495 | 1602 | 12233 |
| <I/σ(I)> | 8.35 | 21.5 | 2.6 |
| Completeness [%] | 99.8 | 85.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.3M sodium malonate pH 7.0, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
