3OUW
Structure of beta-catenin with Lef-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-16 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.97922 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 116.930, 116.930, 214.150 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.940 - 2.910 |
Rwork | 0.203 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | beta-catenin arm domain from beta-catenin/phosphorylated Lef-1 structure |
RMSD bond length | 0.008 |
RMSD bond angle | 1.191 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 103.000 | 3.060 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.102 | 0.396 |
Number of reflections | 15960 | |
<I/σ(I)> | 13 | 2.1 |
Completeness [%] | 96.5 | 82.1 |
Redundancy | 4.9 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 296 | well solution: 4.2% PEG 4000, 100 mM sodium citrate. protein solution: 100 uM beta-cat-arm, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K |