3OUR

Crystal structure of complex between EIIA and a novel pyruvate decarboxylase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-23
Detector	ADSC QUANTUM 270
Wavelength(s)	0.98000
Spacegroup name	C 1 2 1
Unit cell lengths	322.866, 62.067, 126.362
Unit cell angles	90.00, 110.80, 90.00

Refinement procedure

Resolution	49.370 - 2.200
R-factor	0.201
Rwork	0.201
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mve 1o2f
RMSD bond length	0.009
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CCP4
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.230
High resolution limit [Å]	2.190	2.190
Number of reflections	119307
Completeness [%]	98.5	95.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.5	295	20% PEG MME 550, 0.1M MES pH 6.5, 0.01M Zn-Acetate, microbatch, temperature 295K