3OSF

The structure of protozoan parasite Trichomonas vaginalis Myb2 in complex with MRE-2f-13 DNA

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2010-01-30
Detector	Bruker DIP-6040
Wavelength(s)	0.97622, 0.9788, 0.9790, 0.9686
Spacegroup name	P 21 21 21
Unit cell lengths	73.941, 77.352, 84.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.654 - 2.032
R-factor	0.209
Rwork	0.206
R-free	0.25790
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.397
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.070
High resolution limit [Å]	2.030	5.510	2.030
Rmerge	0.047
Number of reflections	31586
Completeness [%]	99.1	95.3	93.1
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	0.1M sodium cacodylate, 15% isopropanol, 25% PEG 4000, 10% glycerol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K