3OS0
PFV strand transfer complex (STC) at 2.81 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 159.859, 159.859, 125.108 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.120 - 2.810 |
| R-factor | 0.20973 |
| Rwork | 0.208 |
| R-free | 0.24056 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3l2q |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.445 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.120 | 2.960 |
| High resolution limit [Å] | 2.810 | 2.810 |
| Rmerge | 0.088 | 0.998 |
| Number of reflections | 39563 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 99.1 | 99.7 |
| Redundancy | 5.7 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 291 | 20% MPD, 40mM MgCl2, 50mM sodium cacodylate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
