3OR1
Crystal structure of dissimilatory sulfite reductase I (DsrI)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2008-08-10 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 114.721, 60.478, 132.915 |
Unit cell angles | 90.00, 94.30, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.760 |
R-factor | 0.193 |
Rwork | 0.193 |
R-free | 0.21200 |
Structure solution method | MAD |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.820 |
High resolution limit [Å] | 1.760 | 1.760 |
Rmerge | 0.071 | 0.381 |
Number of reflections | 179998 | |
<I/σ(I)> | 18.7 | 2.9 |
Completeness [%] | 99.3 | |
Redundancy | 4 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 23%(W/V) PEG6000, 200MM SODIUM CHLORIDE, 100MM MES BUFFER, pH 5.5, VAPOR DIFFUSION, HANGING DROP | |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 23%(W/V) PEG6000, 200MM SODIUM CHLORIDE, 100MM MES BUFFER, pH 5.5, VAPOR DIFFUSION, HANGING DROP |