3OQA
Crystal Structures of Multidrug-Resistant Clinical Isolate 769 HIV-1 Protease Variants
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-D |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-03-22 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 45.136, 45.136, 102.709 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.250 |
| R-factor | 0.20396 |
| Rwork | 0.202 |
| R-free | 0.23778 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tw7 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.826 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.280 | |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 5466 | |
| <I/σ(I)> | 4.8 | |
| Redundancy | 6.4 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.3-1.0M sodium chloride in the pH range 5.5-7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
