3OQ4

Crystal Structure of motif N of Saccharomyces cerevisiae Dbf4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-08-10
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0809
Spacegroup name	P 21 21 2
Unit cell lengths	83.714, 99.707, 127.045
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.408 - 2.400
R-factor	0.2049
Rwork	0.204
R-free	0.22880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3oq0
RMSD bond length	0.007
RMSD bond angle	0.946
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.4_159))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.440
High resolution limit [Å]	2.400	6.510	2.400
Rmerge	0.192	0.152	0.737
Number of reflections	42318
<I/σ(I)>	8.92	9.86	2.6
Completeness [%]	99.8	99.1	100
Redundancy	6.2	5.9	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.1	277	22% MPD (v/v) and 100 mM Na/K phosphate buffer pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 277K