3OQ0
Crystal Structure of motif N of Saccharomyces cerevisiae Dbf4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-02 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9796 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 90.221, 79.358, 127.419 |
Unit cell angles | 90.00, 110.74, 90.00 |
Refinement procedure
Resolution | 35.520 - 2.700 |
R-factor | 0.2066 |
Rwork | 0.205 |
R-free | 0.24410 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.161 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.4_159)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.750 |
High resolution limit [Å] | 2.700 | 7.320 | 2.700 |
Rmerge | 0.100 | 0.055 | 0.795 |
Number of reflections | 46033 | ||
<I/σ(I)> | 16.9 | 19.31 | 2.82 |
Completeness [%] | 99.3 | 98.6 | 99.1 |
Redundancy | 7 | 6.4 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 29% PEG 400, 0.05 M MgCl2, 0.1 M TRIS pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |