3OPT
Crystal structure of the Rph1 catalytic core with a-ketoglutarate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-10 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 64 |
| Unit cell lengths | 109.415, 109.415, 145.958 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.740 - 2.200 |
| R-factor | 0.2003 |
| Rwork | 0.198 |
| R-free | 0.23560 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.631 |
| Data reduction software | HKL-2000 |
| Data scaling software | Diffraction (Anisotropy Server) |
| Phasing software | PHASES |
| Refinement software | PHENIX (1.6.2_432) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.080 | 0.340 |
| Number of reflections | 21926 | |
| <I/σ(I)> | 21.3 | 1.5 |
| Completeness [%] | 77.1 | 39.6 |
| Redundancy | 7.5 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 0.2M ammonium sulfate, 0.1M HEPES, 7% PFG 4000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
