3OPQ
Phosphoribosylaminoimidazole carboxylase with fructose-6-phosphate bound to the central channel of the octameric protein structure.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-13 |
| Detector | MAR CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.590, 96.670, 128.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.191 |
| Rwork | 0.188 |
| R-free | 0.23600 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.477 |
| Data scaling software | XSCALE |
| Phasing software | MrBUMP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.158 | 0.748 |
| Number of reflections | 142585 | |
| <I/σ(I)> | 10.81 | 2.47 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 5.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 295 | 0.2 M NH4 dihydrogen PO4, 0.1 M Tris, 2% Tacsimate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
