3OPE

Structural Basis of Auto-inhibitory mechanism of Histone methyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	110
Detector technology	CCD
Detector	ADSC QUANTUM 210
Wavelength(s)	1.2827
Spacegroup name	P 32 2 1
Unit cell lengths	59.530, 59.530, 233.804
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.900
Rwork	0.235
R-free	0.29940
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.740
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.900	2.900
Rmerge	0.097
Number of reflections	19766
<I/σ(I)>	46.1	4.7
Completeness [%]	99.9	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	Bistrisprofane, PEG, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K